• A compound light microscope magnification is the product of eye and objective lenses, ×10 eyepiece and  ×40 objective, the total magnification is ×400.

Eyepiece

  • Found at the top of the microscope 

  • used to look through the microscope. 

  • It is also named as the ocular. 

  • Its standard magnification is 10x with an optional eyepiece having magnifications from 5X to 30X.

                                           

Eyepiece tube

  •  Is the eyepiece holder.

  •  It carries the eyepiece just above the objective lens

Objective lenses

  • further magnify the specimen mostly they have a magnification power of 4X-100X.

Arm

  • Is a holder connected to all components that function as a support for the microscope so that the microscope can be used properly.

Body tube 

  • Body tube connects the eyepiece to the objective lenses.

                                         

Nose piece

  •  is rotating mount that holds many objective lenses.

  •  It is movable hence it allows to change the magnification.

The Adjustment knobs

  • are used to focus the microscope. 

  • There are two types of adjustment knobs:- 

  • Fine adjustment: regulate the distance between object and objective, to  achieve the necessary sharpness.        

  • Coarse adjustment: 

  • Brings specimen into general focus. 

  • Moves the stage to adjust the difference between the object and the objective. 

  • To capture the exact distance roughly and quickly..

                                             

Stage

  •  is the section in which the specimen is placed for viewing. 

Stage clip

  • acts as a holder for the object plate and ensures that it doesn’t fall out of place accidentally.

Aperture

  • is a hole on the microscope stage, through which the transmitted light from the source reaches the stage.

Microscopic illuminator

  • is the microscopes light source, located at the base. 

  • It is used instead of a mirror. 

  • It captures light from an external source of a low voltage of about 100v.

                                           

Condensers

  • are lenses that are used to collect and focus light from the illuminator into the specimen. 

  • Ensuring clear sharp images are produced with a high magnification of 400X and above. 

Diaphragm

  • known as the iris. 

  • to control the amount of light that reaches the specimen. 

  • It’s an adjustable apparatus, hence controlling the light intensity and the size of the beam of light that gets to the specimen.

Base

  • is the very bottom part. 

  • to accommodate all parts of the light microscope. 

                                       

  • Be very careful when removing the Microscope from the cabinet.

  • Carry the microscope properly, always put your hand beneath its base. 

  • Hold the microscope upright at all times. Do not bump it against anything.

  • Make sure that the slide is clean and dry before putting it on the stage.

  • Put the slide on the stage, with the most promising region exactly in the middle of the hole in the stage that the light comes through. 

  • Always focus on low power (4X objective) first even if eventually you need high power magnification.

  • Focus with the larger coarse-focusing knobs first, then when you have nearly got the image in focus make it really sharp using the smaller fine-focusing knobs.

  • If you want to increase the magnification, move the slide so the most promising region is exactly in the middle of the field of view and then  change to a higher magnification lens.

  • Use immersion oil only with the 100X objective (oil immersion lens) in  place.

  • Use only one drop of oil.

  • Always focus by moving the lens and the specimen further apart, never closer to each other.

  • Never touch the surfaces of the lenses with your fingers or anything else.

  • Lower the stage and then remove the slide when you are done.

  • Always clean the microscope when you are done. (use a lens paper and the alcohol)

  • Always place the 4X objective over the stage and be sure the stage is 

  • at its lowest position before putting the microscope away.

  • Always turn off the light before putting the microscope away.

  • Always return the microscope to the correct cabinet.

  • Always place the oculars toward the back of the cabinet

  • Always wrap the cord correctly before putting 

  • The microscope away.

1.From the parts of microscope, which one found at the top of the microscope and named as the ocular?

A. Nose piece      B. Stage            C. Eyepiece        D. Aperture

2. ____ control the amount of light that reaches the specimen. 

A. Fine adjustment                               B. Coarse adjustment 

C. Diaphragm                                       D. Condenser 

3. One of the following is incorrect handling and using a light microscope.

A. Be very careful when removing the Microscope from the cabinet.

B. Always turn off the light before putting the  microscope away.

C. Use immersion oil only with the 100X objective (oil immersion lens) 

D. Use only one drop of oil.

4. The standard magnification of Eyepiece  is __________ .  

A. 5X                 B.10X                   C. 30X                  D. 40X

5. If the Eye Lens magnification is 5X and the Objective Lens magnification 10X, Total  lens magnification is ________ .

A. 10X                 B. 5X                  C.  50X                   D. 500X 

Answer 

1. C    2.C     3. B     4. B       5. C 

Last modified: Kibxata, 30 Fulbaana 2025, 9:25 AM